The behaviour of wheaten flour towards bakers' and brewers' yeast
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THE CALCIUM BINDING SITES OF THE BAKERS' YEAST TRANSKETOLASE
The calcium binding sites of Bakers' Yeast Transketolase (TK) was elucidated by estimating the pKa values of the functional groups that bind to calcium. These pKa's were found to be 6.25 and 7.2 relating to the pKa's of the two immidazol moieties of histidine residues on the enzyme. The rate of the binding of calcium to the enzyme was obtained separately as a function of pH. Maximum values ...
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AIM To measure circulating antibodies to yeast organisms that could be used to characterise the yeast specific immune response in gastrointestinal disease. METHODS A quantitative, isotype specific enzyme linked immunosorbent assay was developed to measure circulating antibodies to an aqueous extract of Saccharomyces cerevisiae (sacc). Comparisons of specific antibody concentrations were made ...
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Choline kinase was purified approximately 300-fold, in 5% yield, from an autolysate of dried brewers’ yeast. A molecular weight of 67,000 was estimated using a Stokes radius of 33 A, as determined by Sephadex G-200 chromatography. An s~,,,~ of 4.8 S was obtained by sucrose density gradient centrifugation. Enzyme activity was diminished by sulfhydryl inhibitors and stabilized by the presence of ...
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Pseudoporphyria associated with consumption of brewers' yeast.
A case of pseudoporphyria associated with excessive consumption of brewers ' yeast was studied. Detailed analysis of the yeast tablets by high performance liquid chromatography showed the presence of dicarboxylic deuteroporphyrin , mesoporphyrin, and protoporphyrin; coproporphyrin I and III isomers; and uroporphyrin I and III isomers. The faecal porphyrin concentration of the patient taking yea...
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ژورنال
عنوان ژورنال: Journal of the Society of Chemical Industry
سال: 1909
ISSN: 0368-4075,1934-9971
DOI: 10.1002/jctb.5000281405